Publications

Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination
A key challenge when imaging living cells is how to noninvasively extract the most spatiotemporal information possible. Unlike popular …
Planchon, T. A. Gao, L. Milkie, D. E. Davidson, M. W. Galbraith, J. A. Galbraith, C. G. Betzig
DOI
Correlative superresolution light microscopy and electron microscopy
Within cells, the spatial compartmentalization of thousands of distinct proteins serves a multitude of diverse biochemical needs. …
David P Hoffman, Gleb Shtengel, C Shan Xu, Kirby R Campbell, Melanie Freeman, Lei Wang, Dan Milkie, H Amalia Pasolli, Nirmala Iyer, John A Bogovic, Stabley, Daniel R, Shirinifard, Abbas, Pang, Song, David Peale, Kathy Schaefer, Wim Pomp, Chi-Lun Chang, Jennifer Lippincott-Schwartz, Tom Kirchhausen, David J Solecki, Eric Betzig, Harald F Hess
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Correlative superresolution light microscopy and electron microscopy
An adaptive optics module for deep tissue multiphoton imaging in vivo
Achieving mouse brain in vivo subcellular resolution deeper than ever before by combining adaptive optics with 2P and 3P.
Cristina Rodrı́guez, Anderson Chen, José A Rivera, Manuel A Mohr, Yajie Liang, Wenzhi Sun, Dan Milkie, Thomas G Bifano, Xiaoke Chen, Na Ji
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An adaptive optics module for deep tissue multiphoton imaging in vivo
US Patent. High-Resolution, Real-Time Imaging With Adaptive Optics and Lattice Light Sheets
Robert Eric Betzig, Tsung-li Liu, Dan Milkie, Kai Wang, Wesley Legant
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Expansion Microscopy + Lattice Lightsheet for rapid mouse & fly brain synaptic imaging
Optical and electron microscopy have made tremendous inroads toward understanding the complexity of the brain. However, optical …
Ruixuan Gao, Shoh M Asano, Srigokul Upadhyayula, Pisarev Igor, Dan Milkie, Tsung-li Liu, Singh Ved, Graves Austin, Grace H Huynh, Yongxin Zhao, John Bogovic, Jennifer Colonell, Carolyn M Ott, Christopher Zugates, Susan Tappan, Alfredo Rodriguez, Kishore R Mosaliganti, Shu-Hsien Sheu, H Amalia Pasolli, Song Pang, C Shan Xu, Sean G Megason, Harald Hess, Jennifer Lippincott-Schwartz, Adam Hantman, Gerald M Rubin, Tom Kirchhausen, Stephan Saalfeld, Yoshinori Aso, Edward S Boyden, Eric Betzig
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Expansion Microscopy + Lattice Lightsheet for rapid mouse & fly brain synaptic imaging
Visualizing intracellular organelle and cytoskeletal interactions at nanoscale resolution on millisecond timescales
In eukaryotic cells, organelles and the cytoskeleton undergo highly dynamic yet organized interactions capable of orchestrating complex …
Yuting Guo, Di Li, Siwei Zhang, Yanrui Yang, Jia-Jia Liu, Xinyu Wang, Chong Liu, Dan Milkie, Regan P Moore, U Serdar Tulu, others
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Visualizing intracellular organelle and cytoskeletal interactions at nanoscale resolution on millisecond timescales
TEM imaging of an entire fly brain

A full adult fly brain (FAFB), imaged at synaptic resolution using TEM.

  • 7,062 brain slices
  • 23 million images
  • 115 TB on disk
  • Over 1.5 years
  • 1/5 acquired autonomously with custom developed Fast Stage Autoloader robot.
Zhihao Zheng, J Scott Lauritzen, Eric Perlman, Camenzind G Robinson, Matthew Nichols, Dan Milkie, Omar Torrens, John Price, Corey B Fisher, Nadiya Sharifi, Calle-Schuler, Steven A, Kmecova, Lucia, Ali, Iqbal J, Karsh, Bill, Trautman, Eric T, Bogovic, John A, Hanslovsky, Philipp, Jefferis, Gregory S X E, Kazhdan, Michael, Khairy, Khaled, Saalfeld, Stephan, Fetter, Richard D, Bock, Davi D
Cite Dataset DOI Surf the data
TEM imaging of an entire fly brain
Lattice Lightsheet + AO
True physiological imaging of subcellular dynamics requires studying cells within their parent organisms, where all the environmental …
Tsung-li Liu, Srigokul Upadhyayula, Dan Milkie, Ved Singh, Kai Wang, Ian A Swinburne, Kishore R Mosaliganti, Zach M Collins, Tom W Hiscock, Jamien Shea, others
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Lattice Lightsheet + AO
3D P.A.I.N.T. Microscopy
Extending three-dimensional (3D) single-molecule localization microscopy away from the coverslip and into thicker specimens will …
Wesley R Legant, Lin Shao, Jonathan B Grimm, Timothy A Brown, Dan Milkie, Brian B Avants, Luke D Lavis, Eric Betzig
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3D SIM super-resolution microscopy
Super-resolution fluorescence microscopy is distinct among nanoscale imaging tools in its ability to image protein dynamics in living …
Dong Li, Lin Shao, Bi-Chang Chen, Xi Zhang, Mingshu Zhang, Brian Moses, Dan Milkie, Jordan R Beach, John A Hammer, Mithun Pasham, others
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3D SIM super-resolution microscopy
Lattice Light Sheet microscopy
Although fluorescence microscopy provides a crucial window into the physiology of living specimens, many biological processes are too …
B. C. Chen, W. R. Legant, K. Wang, L. Shao, Dan Milkie, M. W. Davidson, C. Janetopoulos, X. F. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, E. Betzig
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Lattice Light Sheet microscopy
Two-photon, confocal + AO
Using a descanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical …
Kai Wang, Dan Milkie, Ankur Saxena, Peter Engerer, Thomas Misgeld, Marianne E Bronner, Jeff Mumm, Eric Betzig
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Two-photon, confocal + AO
Multiplexed aberration measurement for deep tissue imaging in vivo
We describe an adaptive optics method that modulates the intensity or phase of light rays at multiple pupil segments in parallel to …
Chen Wang, Rui Liu, Dan Milkie, Wenzhi Sun, Zhongchao Tan, Aaron Kerlin, Tsai-Wen Chen, Douglas S Kim, Na Ji
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Direct phase measurement in zonal wavefront reconstruction using multidither coherent optical adaptive technique
In traditional zonal wavefront sensing for adaptive optics, after local wavefront gradients are obtained, the entire wavefront can be …
Rui Liu, Dan Milkie, Aaron Kerlin, Bryan MacLennan, Na Ji
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Pupil-segmentation-based adaptive optical microscopy with full-pupil illumination
Optical aberrations deteriorate the performance of microscopes. Adaptive optics can be used to improve imaging performance via …
Dan Milkie, Eric Betzig, Na Ji
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Optical, magnetic, and electrical properties of single-walled carbon nanotubes
Dan Milkie
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Measurement of chiral-dependent magnetic anisotropy in carbon nanotubes
The magnetic properties of small diameter semiconducting single-walled carbon nanotubes have been recently predicted to depend …
Omar N Torrens, Dan Milkie, Han Y Ban, Ming Zheng, G Bibiana Onoa, Timothy D Gierke, James M Kikkawa
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Composites reinforced with carbon nanotubes enhances strength and properties
The creation of carbon nanotube (CNT) aerogels from aqueous-gel precursors by critical-point-drying and freeze-drying is reported. The …
Mateusz B Bryning, Dan Milkie, Mohammad F Islam, Lawrence A Hough, James M Kikkawa, Arjun G Yodh
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Composites reinforced with carbon nanotubes enhances strength and properties
Photoluminescence from intertube carrier migration in single-walled carbon nanotube bundles
Photoluminescence (PL) identifies spectroscopic signatures of intertube transfer of optically pumped carriers in single-walled carbon …
ON Torrens, Dan Milkie, M Zheng, JM Kikkawa
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Thermal conductivity and interfacial resistance in single-wall carbon nanotube epoxy composites
We report thermal conductivity measurements of purified single-wall carbon nanotube (SWNT) epoxy composites prepared using suspensions …
MB Bryning, Dan Milkie, MF Islam, JM Kikkawa, AG Yodh
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Magnetic heterogeneity and alignment of single wall carbon nanotubes
Magnetic alignment studies demonstrate that a fraction of acid purified single wall carbon nanotubes (SWNTs) exhibit both …
MF Islam, Dan Milkie, ON Torrens, AG Yodh, JM Kikkawa
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Controlled switching of optical emission energies in semiconducting single-walled carbon nanotubes
We present scanning photoluminescence (PL) microscopy of freely suspended single-walled carbon nanotubes grown by chemically assisted …
Dan Milkie, C Staii, S Paulson, E Hindman, AT Johnson, JM Kikkawa
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Direct measurement of the polarized optical absorption cross section of single-wall carbon nanotubes
We determine optical absorption cross sections of single-wall carbon nanotubes for visible light copolarized and cross polarized with …
MF Islam, Dan Milkie, CL Kane, AG Yodh, JM Kikkawa
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